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| 优势产品 |
| Wizard® Plus SV Minipreps DNA Purification Systems |
| 2006-04-26 15:47:19 |
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(已经被浏览794次)
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| Available Separately |
| For Laboratory Use.
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| Component Listing
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| Description |
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The Wizard® Plus SV Minipreps DNA Purification System,(a,b) a silica membrane-based system, provides a simple and reliable method for rapid isolation of plasmid DNA. The entire miniprep procedure can be completed in 45 minutes or less, depending on the number of samples processed. Using the system, plasmid DNA can be purified from 1–10ml of overnight E. coli culture. The purified plasmid DNA can be used directly for automated fluorescent DNA BigDye® terminator sequencing as well as for other standard molecular biology techniques without further manipulation. It also can be used for in vitro transcription reactions when supplemented with a ribonuclease inhibitor such as Recombinant RNasin® Ribonuclease Inhibitor (Cat.# N2511).
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| Features |
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- Improve Productivity: Isolate 20 minipreps in less than 45 minutes.
- High Performance: 1–20μg of high-quality plasmid DNA, enough for multiple applications.
- Safe and Convenient: No phenol extractions or precipitations required.
- Flexible: Choice of spin (microcentrifuge) or vacuum purification formats.
- Consistent Quality: Alkaline protease step improves plasmid quality.
- Confidence in Results: Purified DNA meets a target of >98% accuracy over 500 bases using pGEM®-3Zf(+) Vector in BigDye® terminator sequencing.
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| Applications |
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For more information, see the Protocols & Applications Guide.
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| Protocol |
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Technical Bulletin #TB225.
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| FAQ |
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Wizard® Plus SV Minipreps DNA Purification System FAQ.
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| Storage Conditions |
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Store at 22–25°C.
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| Patents/Disclaimers |
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(a)U.S. Pat. No. 5,981,235, Australian Pat. No. 729932 and other patents and patents pending.
(b)Australian Pat. No. 730718 and other patents and patents pending.
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