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| 优势产品 |
| PowerPlex® Y System |
| 2006-04-26 16:20:51 |
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(已经被浏览920次)
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| Available Separately |
| Not for Medical Diagnostic Use.
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| Component Listing
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| Description |
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The PowerPlex® Y System(a) allows co-amplification and three-color detection of twelve loci. The system contains primers for the loci DYS19, DYS385a/b, DYS389I/II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438 and DYS439. One primer specific for DYS389I/II, DYS391 and DYS439 is labeled with fluorescein (FL); one primer specific for DYS385a/b, DYS390 and DYS393 is labeled with carboxy-tetramethylrhodamine (TMR); and one primer specific for DYS19, DYS392, DYS437 and DYS438 is labeled with 6-carboxy-4´,5´-dichloro-2´,7´-dimethoxy-fluorescein (JOE).
All twelve loci are amplified simultaneously in a single tube and analyzed in a single injection or gel lane. Fragment sizing is provided by an internal size standard (Internal Lane Standard 600) labeled with carboxy-X-rhodamine (CXR). Color deconvolution can be performed with color matrix kits available from Promega.
The PowerPlex® Y System is compatible with the ABI PRISM® 310, 3100, 3100-Avant, 3130 and 3130xl Genetic Analyzers and ABI PRISM® 377 DNA Sequencer.
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| Features |
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- Amplification of 12 Y-STR Loci in One Reaction: Amplify the nine loci defined as the European minimal haplotype and endorsed by ISFG (International Society of Forensic Genetics)* plus the two loci added to this panel by SWGDAM (Scientific Working Group on DNA Analysis Methods).
*For more information on the European minimal haplotype, go to: Y-STR Haplotype Reference Database. ISFG-endorsed loci are DYS19, DYS385a/b, DYS389I/II, DYS390, DYS391, DYS392, DYS393. SWGDAM-endorsed loci are DYS19, DYS385a/b, DYS389I/II, DYS390, DYS391, DYS392, DYS393, DYS438 and DYS439.
- Maximum Sensitivity: The system is optimized for use with 0.5–1ng of template DNA. However, each lot is performance tested to provide reproducible results with 0.25ng of DNA. Additional studies show interpretable results can be obtained with <0.25ng of DNA.
- Superior Specificity—You Amplify Only Male DNA: Each lot is performance tested to ensure no amplification with up to 100ng of female DNA. Additional studies show no amplification with >100ng of female DNA. This makes the PowerPlex® Y System a great choice to analyze "heavy" mixture samples.
- Short PCR Products: Amplification products are all less than 335 bases, which increases the likelihood of obtaining a full profile from degraded samples.
- No Locus Overlap: Primers have been designed so that alleles for each locus do not overlap with a neighboring locus, reducing the likelihood of misinterpretation.
- Typing of Samples with Greater Confidence: Allelic ladder for all 12 loci are provided to simplify interpretation through the use of the PowerTyper™ Macro. The wide span of alleles included in the ladder allow typing of more rare alleles.
- Automatic Assignment of Genotypes: The PowerTyper™ Y Macro (available separately) has been designed to automatically label fragments generated from Genotyper® data. The PowerTyper™ Macros can be downloaded from: Genetic Identity Tools or ordered on CD-ROM. Panel and bin files for use with GeneMapper® ID are available for download at: Genetic Identity Tools
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| Protocol |
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PowerPlex® Y System Technical Manual #TMD018.
PowerPlex® Matrix Standards, 3100, Technical Bulletin #TBD019.
PowerPlex® Matrix Standards, 310/377, Technical Bulletin #TBD018.
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| Storage Conditions |
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Store at –20°C. The fluorescent primer pairs and allelic ladders are light-sensitive; therefore, minimize light exposure.
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| Patents/Disclaimers |
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(a)STR loci are the subject of U.S. Pat. No. RE 37,984, German Pat. No. DE 38 34 636 C2 and other patents issued to the Max-Planck-Gesellschaft zur Förderung der Wissenschaften, e.V., Germany. The development and use of STR loci are covered by U.S. Pat. No. 5,364,759, Australian Pat. No. 670231 and other pending patents assigned to Baylor College of Medicine, Houston, Texas.
Patents for the foundational PCR process, European Pat. Nos. 201,184 and 200,362, expired on March 28, 2006. In the U.S., the patents covering the foundational PCR process expired on March 29, 2005.
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